Inventors : Mr. Janakiram Chodavarapu &
Dr. M. Khalilullah

Example 1 Solvent Extraction of the Roots

The roots of Dodonaea petiolaris and Dodonaea viscosa were removed from the plant, dried and then ground into powder. The powdered roots were then extracted successively by conventional methods with a series of organic solvents (n-hexane, petroleum ether, ethylacetate, acetone, (95%) ethanol, diethyl ether, benzene and toluene). The organic solvents were recovered by distillation and the residues thus obtained were mixed and termed as an absolute for incorporation into the compositions of the present invention.

Example 2 Solvent Extraction of the Bark

The bark of Dodonaea petiolaris and Dodonaea viscosa was removed from the plant, dried and then ground into a coarse powder. The powdered bark was then extracted successively by conventional methods with a series of organic solvents (n-hexane, petroleum ether, ethylacetate, acetone, (95%) ethanol, diethyl ether, benzene and toluene). The organic solvents were recovered by distillation and the residues thus obtained were mixed and termed as an absolute for incorporation into the compositions of the present invention or for recombination with the absolute obtained from Example 1.

Example 3 Solvent Extraction of the Leaves

The leaves of Dodonaea petiolaris and Dodonaea viscosa were removed from the plant, dried and then ground into powder. The powdered roots were then extracted successively by conventional methods with a series of organic solvents (n-hexane, petroleum ether, ethylacetate, acetone, (95%) ethanol, diethyl ether, benzene and toluene) at their respective boiling points. The organic solvents were recovered by distillation. Traces of solvents were removed under mild vacuum. An oily residue was thus obtained for incorporation into the compositions of the present invention or for recombination with the absolute obtained from Example 1 or the recombination obtained from Example 2.

Example 4 Solvent Extraction of the Seeds

The seeds of Dodonaea petiolaris and Dodonaea viscosa were picked during the months from October to January in India. The seeds were dried and then crushed. The powdered seeds were then extracted successively by conventional methods with a series of organic solvents (n-hexane, petroleum ether, ethylacetate, acetone, (95%) ethanol, diethyl ether, benzene and toluene). The organic solvents were recovered by distillation. Traces of solvents were removed under mild vacuum. A yellow, oily residue was thus obtained for incorporation into the compositions of the present invention or for recombination with the absolute obtained from Example 1 or the recombinations obtained from Examples 2 and 3.

Example 5

As described in Examples 1 through 4 above, an extract was prepared via solvent extraction of the separated leaves, roots, bark and seeds of Dodonaea petiolaris and Dodonaea viscosa, followed by recombination of the four extracts thus obtained. This recombined residue was again subjected to solvent extraction with 95% ethanol. The solvent was recovered by distillation and traces of the solvent were removed under mild vacuum, yeilding a concentrated absolute. The absolute thus obtained can then be subjected to oxidation under conditions which both include and exclude an oxidizing agent.

Example 6

The absolute obtained in Example 5 was oxidized by exposure to gaseous oxygen for about 20 hours at an ambient temperature of about 35 to 40 degrees celsius.

Example 7

The absolute obtained in Example 5 was oxidized by exposure to a free current of air at ambient temperature (about 35 to 40 degrees celsius) for about 744 hours with continuous stirring.

Example 8

The absolute obtained in Example 5 was oxidized by exposure to a free current of air at a temperature of about 85 to 90 degrees celsius for about 72 hours with continuous stirring.